Establishment Of An Efficient In Vitro Regeneration System Of Ridge Gourd (Luffa Acutangula L. Roxb) From Immature Embryo And Cotyledon Explants

Ridge gourd (Luffa acutangula L. Roxb) is a popular and nutritious vegetable in Bangladesh. The present investigation was undertaken to establish an efficient in vitro regeneration system of ridge gourd from immature embryo and cotyledon explants. Immature embryos and cotyledons were collected from immature seeds and culture on MS medium supplemented with various concentration and combination of growth regulators for primary development and multiple shoot formation. The highest result of direct shoot multiplication was observed from immature embryo and cotyledon on MS with 1.0 mg/l BAP + 0.2 mg/l GA3 and 2.0 mg/l Kin + 0.3 mg/l GA3 respectively. The highly regenerative callus with light green compact structure was obtained from cotyledon explants using 1.5 mg/l BAP + 1.0 mg/l NAA and effective regeneration from callus was found on MS medium supplemented 3.5 mg/l BAP + 0.2 mg/l GA3. In vitro regenerated shoots were subcultured on 1⁄2 strength MS medium containing different concentrations of IBA and NAA for successful root induction and the efficient result was found using 0.5 mg/l IBA.